This is a test reported by oama on ReefCentral.

This freshwater test can help determine if a fish is infected with Amyloodinium. Additionally, if the fish is infected with this parasite, the freshwater dip will remove many of the trophonts.

The test should be performed in a clear or nearly clear bowl or dish, just deep enough for the fish. Distilled, Reverse Osmosis or Reverse Osmosis/Deionised water should be used. It is a good idea to aerate the water for 30 minutes or so to ensure it has sufficient oxygen. Some processed water can be a little low on oxygen. The temperature of the freshwater should be as close as possible to the temperature of the tank water. The pH of the freshwater should be as close as possible to the pH of the tank water. Add a small amount of baking soda (around half a teaspoon) to the water and mix well. This will add some bicarbonate and carbonate to the water which will help with the pH.

Place the fish in the freshwater for around 3 minutes. Make sure the fish does not get too stressed and if it shows signs of extreme stress, remove it immediately. After 3 minutes put the fish back into the tank and inspect the water in the dish closely. Swirl the water around. If you see what looks like dust or powder in the water, it is likely to be the Amyloodinium trophonts that have detached from the fish. If the water is clear with no dust or powder, either the fish doesn't have Amyloodinium or the trophonts have been attached for less than 24 hours.

Last updated: May 6, 2004