This is my recommended process for establishing a new tank. It is designed to increase the chances of the survival of organisms introduced at various stages during the process. The process assumes a deep sand bed will be used. If a deep sand bed is going to be used remotely, for example in a sump or refugium, the process can still be followed, but the steps relating to the sand bed should be carried out in the remote sand bed. If no sand bed is to be used, a different approach will be required as there will be limited surface area for bacteria to colonise until the first live rock is added.

  1. Place the sand in the bottom of the tank to a depth of at least 10 cm (4").

    Use sand with an average grain size of 0.125mm, but with mixed grains.

    The sand bed will be the initial biological filter for the tank and will reduce ammonia levels at later stages of the process so that added organisms have a better chance of survival.

  2. Fill the tank with seawater. Start the sump return and in tank circulation. Bring the water to the desired temperature (26-28°C 79-82°F) and specific gravity (1.025-1.028).

    While not necessary initially, running the return and in tank circulation will ensure the water is well oxygenated which will help to establish the necessary populations of nitrifying bacteria. Similarly, providing normal reef temperatures and specific gravity will mean less change later which can effect any organisms in the tank.

  3. Feed the tank as you would if you had 1 or 2 fish and continue to feed every 2-3 days.

    If you wish, monitor the water for ammonia and nitrite. When the nitrite has been at zero for at least a week, move to the next step. Using this method you may not register any ammonia or nitrite. If you don't, that's fine, just wait around 3 weeks after starting before moving to the next step.

    The food is added to provide ammonia to start the populations of bacteria that oxidise ammonia to nitrite. The nitrite will provide for bacteria that oxidise nitrite to nitrate. Adding small amounts of food results in relatively less ammonia than adding something like a whole prawn or shrimp. As high levels of ammonia can inhibit the bacteria that oxidise nitrite, spiking ammonia can extend the period over with the necessary populations of bacteria are established. Additionally, the populations required to nitrify the remains of a prawn or shrimp may be more than is required and the populations may take longer to stabilise.

  4. Start the skimmer.

  5. Seed the tank with live sand.

    If you live in a warm temperate to tropical area you can collect your own live sand for seeding the tank. You don't need a lot of sand, but collect it from as many places as possible and also get some from other people's tanks. What you want is a diversity of organisms.

  6. Feed the tank as if you had 3 or 4 fish in the tank.

    This will provide food for the sand bed organisms allowing them to reproduce and populate the sand bed. I feel it is important to have the sand bed populated with sand bed organisms rather than ones from the live rock.

  7. Wait 3-8 weeks for the organisms in the sand bed to reproduce.

    During this period you can enjoy watching all the new organisms appearing in the sand bed.


    Burrows made by polychaetes in a new sand bed that has just been seeded with live sand

  8. Add you first pieces of live rock. Start with around 5kg/12 lbs and wait a couple of weeks.

    It is good to start with a small amount of live rock first in case there is any die off of organisms on the rock. The added rock will provide more surface area for filtration bacteria and so help when more live rock is added later.

  9. Add the next lot of live rock. Add about half of what you are planning to add (minus the 5 kg/12 lbs you have already added). Wait a couple more weeks.

    As with the first batch of live rock, adding a relatively small amount will maximise survival of organisms on and in the live rock.

  10. Add the rest of the live rock and aquascape the tank to your liking.

  11. Wait a week or so, or monitor the ammonia and nitrite.

  12. If ammonia and nitrite have been zero for at least a week, add some snails (2-3 different species) and a sea urchin.

    It is best to have the herbivores in the tank before you start having problems with algae.

    You may not have detectable ammonia or nitrate during the much of the process because biological filtration was established early on. However, it is still best to wait at least a week after completing this step to ensure some stability in the biological filtration capacity of the tank. Note that while nitrite is not very toxic in seawater, presence suggests instability of the biological filtration.

  13. You tank is now ready for you to add other organisms.

This will take some patience, but you will be rewarded in the end.


Last updated: January 11, 2005